Purification and Characterization of Protease Enzyme from Oleander (Nerium oleander) Flowers of Different Colors
Keywords:
Oleander (Nerium oleander), Purification, Protease, CharacterizationAbstract
In this study, protease enzyme was purified and characterized from oleander (Nerium oleander) flowers collected from Mugla countryside in June-September period.
Protease catalyzes the hydrolysis of proteins to peptides and amino acids. It is one of the most important enzyme groups in both industrial and biochemical applications. Using the TPP method, the protease enzyme from oleander flowers (white, pink and red) was obtained in order of high efficiency.
Optimum pH and optimum temperature for enzyme, KM and Vmax values for casein substrates were determined. SDS-PAGE was used to control the purity of the protease enzyme purified from oleander flowers. The molecular weight of protease enzyme purified from oleander flowers was determined by gel filtration chromatography to be white oleander 40,537 kea pink oleander 21,386 kDa and 22,516 kDa.
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